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with a number of substrate analogs. The response mechanism is of desire considering the fact that NagA from bacterial sources is discovered with either a couple of divalent metallic ions during the Lively internet site.
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m is one/four hundred of that calculated for that wild form enzyme. These final results affirm the worth for the purpose of H251 in the binding of substrate throughout the Energetic internet site of NagA. The homologous residue was observed to interact with d-glucosamine-6-phosphate while in the structure of NagA from B. subtilis
m for the E131Q mutant is greater to 3.six mM. A significant portion of the reduction in catalytic efficiency has arisen because the metallic articles with the purified proteins is comparatively reduced.
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